HREM: High-Resolution Episcopic Microscopy
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چکیده
The resin blocks are mounted on a motorized microtome. The microtome has to be modified to allow the block holder to reproducibly come to rest at a "photoposition" after each cut. E.g., the apparatus we operate in Vienna has its photoposition at the upper stopping point of the block holder excursion of a rotary microtome (Microtec CUT 4060E, microTec Laborgeräte GmbH). It is reproducibly reached with an accuracy of approximately +/1 μm.
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Embedding embryos for high-resolution episcopic microscopy (HREM).
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